Cov tshuaj tua kab lossis fungicidal toxicity ntawm cov tshuaj tua kab feem ntau yog qhia raws li "qhov nruab nrab lethal koob" (LD50), uas yog qhov koob tshuaj (mg / kg) yuav tsum tua ib nrab (50%) ntawm cov pejxeem lom. Yog hais tias qhov concentration yog qhia raws li koob tshuaj, nws yog hu ua "median lethal concentration" (LC50). Cov tshuaj fungicides tau nthuav tawm raws li ED50 lossis EC50, uas yog cov koob tshuaj lossis cov ntsiab lus xav tau los inhibit 50% spore germination.
Spore Germination Assay: Cov tshuaj tua kab sib txawv yog txau rau ntawm iav swb lossis phaj. Ntxiv ib qho quantitative spore suspension. Tom qab kev sib cuag nrog cov tshuaj tua kab thiab ib lub sij hawm incubation, qhov feem pua ntawm spores germination yog soj ntsuam nyob rau hauv ib lub microscope.
Inhibition Zone Method: Kev ncua ntawm cov kab mob fungal spores lossis hyphae yog tov nrog agar nruab nrab. Tom qab txias, sterilized voj voog lim ntawv discs (kwv yees li 6 hli nyob rau hauv txoj kab uas hla) dipped nyob rau hauv sib txawv concentrations ntawm tshuaj tua kab yog muab tso rau saum npoo ntawm nruab nrab. Tom qab incubation ntawm qhov kub thiab txias rau ib lub sij hawm, qhov kev loj hlob ntawm cov kab mob yog inhibited vim lub diffusion ntawm cov tshuaj tua kab, tsim ib tug "inhibition cheeb tsam." Qhov loj ntawm cheeb tsam inhibition yog ntsuas los sib piv cov tshuaj toxicity ntawm fungicide.
Kev loj hlob tus nqi: Cov tshuaj tov yog ntxiv rau agar nruab nrab, thiab tom qab condensation, cov kab mob yog inoculated. Tom qab 24-48 teev, kev loj hlob hauv cheeb tsam raug pom, qhov kev loj hlob yog xam, thiab muab piv nrog kev loj hlob ntawm pawg tswj tsis muaj tshuaj.